The brokerage fee will be deducted from the Autoinvest amount you selected. The brokerage fee will be included in each Autoinvest transaction, unless you purchase free shares.
An example of how the brokerage fee is deducted:
Autoinvest scheduled: $100
Brokerage fee: $9.50
Stock price: $84.02
Remaining cash in account: $6.48
The remaining cash will be included in your next Autoinvest transaction.